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Canto reported an excellent summary and discussion of all techniques reported to date on the aging of ink (11).
Brunelle (7,8) and Aginaky (9) have since responded on the use of accelerated aging of ink to estimate the age of inks on questioned documents and both have used their technique on actual cases. Brunelle measures the extraction of ink dye components into solvents.
For all the inks tested to date, these measurements detect changes in ink up to approximately 6 years.
Samples taken for accelerated aging were heated in a temperature controlled oven for 30 minutes at 100 degrees C.
Then, these samples were allowed to equilibrate at room temperature for 30 minutes before performing the solvent extractions.
After the plate cooled to room temperature, the TLC plate containing the ink spots was scanned in the scanning TLC densitometer, which recorded the relative concentrations of each spot on the plate in relative percentages.